mouse monoclonal anti arc Search Results


mouse anti-arc monoclonal antibody  (Becton Dickinson)
90
Becton Dickinson mouse anti-arc monoclonal antibody
Mouse Anti Arc Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti+arc/pmc06673172-360-7-12?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-arc monoclonal antibody - by Bioz Stars, 2026-08
90/100 stars
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anti–p16-arc  (Synaptic Systems)
90
Synaptic Systems anti–p16-arc
CLIP-170 function is important for actin polymerization and mDia1 recruitment. (A) RAW264.7 macrophages were transfected with pSUPER-AB (directed against CLIP-170 and CLIP-115 [left]) or pEGFP–CLIP-170ΔH (right) and then allowed to phagocytose C3bi-SRBCs for 10 min at 37°C. The cells were fixed and stained with AMCA– or Cy2–anti-rabbit IgG to detect the external particles, Alexa 488– or Alexa 350–phalloidin to stain F-actin, and anti-mDia1 or anti–CLIP-170 antibodies followed by Cy3–anti-mouse IgG. 50 CLIP-170–inhibited cells and 50 control cells were scored for the presence or absence of F-actin or mDia1 accumulation around bound particles. Results are expressed as a percentage of control cells. Means ± SEM of three independent experiments are plotted. *, P < 0.001; **, P < 0.005. (B) RAW264.7 macrophages were allowed to phagocytose C3bi-SRBCs for 10 min at 37°C, fixed, permeabilized, and incubated with Alexa 546–phalloidin. Images are shown using the Grayscale (left) or Rainbow2 (right) look-up tables. Values on the color scales in the right corner of the right panel indicate the fluorescence intensities. Bar, 10 μm. (C) The profiles of F-actin fluorescence intensities along the line drawn at the phagocytic site (line 1) and in the cell body (line 2) are shown. (D) Macrophages were transfected with pEGFP–CLIP-170ΔH or pEGFP as a control and then phagocytosis was performed as in B. mDia1 was detected with specific antibodies and the Arp2/3 complex was stained with <t>anti-p16</t> antibodies, each followed by Cy3-F(ab′) 2 anti-mouse IgG antibodies. The fluorescence intensities measured in the phagocytic cups were background subtracted and expressed as a percentage of the intensities in the cell body. Data obtained for F-actin (left), mDia1 (middle), and Arp2/3 (right) were plotted. Data are the mean ± SEM of three independent experiments. 15 CLIP-170ΔH–expressing and 15 GFP-expressing control cells were analyzed in each experiment. *, P < 0.005. Enrichment of Arp2/3 was not significantly impaired (P > 0.1).
Anti–P16 Arc, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti+arc/pmc02606960-185-45-46?v=Synaptic+Systems
Average 90 stars, based on 1 article reviews
anti–p16-arc - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
anti-arg 3.1 (arc) mouse monoclonal antibody  (Boster Bio)
N/A
Boster Bio ARC mouse monoclonal antibody,clone OTI2B3. Catalog# M08455. Tested in WB. This antibody reacts with Human, Mouse, Rat.
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Image Search Results


CLIP-170 function is important for actin polymerization and mDia1 recruitment. (A) RAW264.7 macrophages were transfected with pSUPER-AB (directed against CLIP-170 and CLIP-115 [left]) or pEGFP–CLIP-170ΔH (right) and then allowed to phagocytose C3bi-SRBCs for 10 min at 37°C. The cells were fixed and stained with AMCA– or Cy2–anti-rabbit IgG to detect the external particles, Alexa 488– or Alexa 350–phalloidin to stain F-actin, and anti-mDia1 or anti–CLIP-170 antibodies followed by Cy3–anti-mouse IgG. 50 CLIP-170–inhibited cells and 50 control cells were scored for the presence or absence of F-actin or mDia1 accumulation around bound particles. Results are expressed as a percentage of control cells. Means ± SEM of three independent experiments are plotted. *, P < 0.001; **, P < 0.005. (B) RAW264.7 macrophages were allowed to phagocytose C3bi-SRBCs for 10 min at 37°C, fixed, permeabilized, and incubated with Alexa 546–phalloidin. Images are shown using the Grayscale (left) or Rainbow2 (right) look-up tables. Values on the color scales in the right corner of the right panel indicate the fluorescence intensities. Bar, 10 μm. (C) The profiles of F-actin fluorescence intensities along the line drawn at the phagocytic site (line 1) and in the cell body (line 2) are shown. (D) Macrophages were transfected with pEGFP–CLIP-170ΔH or pEGFP as a control and then phagocytosis was performed as in B. mDia1 was detected with specific antibodies and the Arp2/3 complex was stained with anti-p16 antibodies, each followed by Cy3-F(ab′) 2 anti-mouse IgG antibodies. The fluorescence intensities measured in the phagocytic cups were background subtracted and expressed as a percentage of the intensities in the cell body. Data obtained for F-actin (left), mDia1 (middle), and Arp2/3 (right) were plotted. Data are the mean ± SEM of three independent experiments. 15 CLIP-170ΔH–expressing and 15 GFP-expressing control cells were analyzed in each experiment. *, P < 0.005. Enrichment of Arp2/3 was not significantly impaired (P > 0.1).

Journal: The Journal of Cell Biology

Article Title: The microtubule-binding protein CLIP-170 coordinates mDia1 and actin reorganization during CR3-mediated phagocytosis

doi: 10.1083/jcb.200807023

Figure Lengend Snippet: CLIP-170 function is important for actin polymerization and mDia1 recruitment. (A) RAW264.7 macrophages were transfected with pSUPER-AB (directed against CLIP-170 and CLIP-115 [left]) or pEGFP–CLIP-170ΔH (right) and then allowed to phagocytose C3bi-SRBCs for 10 min at 37°C. The cells were fixed and stained with AMCA– or Cy2–anti-rabbit IgG to detect the external particles, Alexa 488– or Alexa 350–phalloidin to stain F-actin, and anti-mDia1 or anti–CLIP-170 antibodies followed by Cy3–anti-mouse IgG. 50 CLIP-170–inhibited cells and 50 control cells were scored for the presence or absence of F-actin or mDia1 accumulation around bound particles. Results are expressed as a percentage of control cells. Means ± SEM of three independent experiments are plotted. *, P < 0.001; **, P < 0.005. (B) RAW264.7 macrophages were allowed to phagocytose C3bi-SRBCs for 10 min at 37°C, fixed, permeabilized, and incubated with Alexa 546–phalloidin. Images are shown using the Grayscale (left) or Rainbow2 (right) look-up tables. Values on the color scales in the right corner of the right panel indicate the fluorescence intensities. Bar, 10 μm. (C) The profiles of F-actin fluorescence intensities along the line drawn at the phagocytic site (line 1) and in the cell body (line 2) are shown. (D) Macrophages were transfected with pEGFP–CLIP-170ΔH or pEGFP as a control and then phagocytosis was performed as in B. mDia1 was detected with specific antibodies and the Arp2/3 complex was stained with anti-p16 antibodies, each followed by Cy3-F(ab′) 2 anti-mouse IgG antibodies. The fluorescence intensities measured in the phagocytic cups were background subtracted and expressed as a percentage of the intensities in the cell body. Data obtained for F-actin (left), mDia1 (middle), and Arp2/3 (right) were plotted. Data are the mean ± SEM of three independent experiments. 15 CLIP-170ΔH–expressing and 15 GFP-expressing control cells were analyzed in each experiment. *, P < 0.005. Enrichment of Arp2/3 was not significantly impaired (P > 0.1).

Article Snippet: The following antibodies were used: anti–CLIP-170 (clone F-3; Santa Cruz Biotechnology, Inc.); #2221 serum (a gift from N. Galjart, Erasmus University, Rotterdam, Netherlands); anti-EB3 serum (a gift from A. Akhmanova, Erasmus University); anti–α-tubulin (DM1A; Sigma-Aldrich); anti-myc (Roche); anti-mDia1, anti-EB1, anti-CLIP-115, and anti-clathrin heavy chain (BD); anti–p16-Arc (Synaptic Systems GmbH); anti-GST (home made); anti-tubulin (clone F2C; ; ); purified rabbit anti-SRBC (IGN Biochemicals); purified rabbit anti-SRBC IgM (Accurate); AMCA-, Cy2-, Cy3-, or Cy5-labeled F(ab′) 2 anti-mouse, -human, and -rabbit (Jackson ImmunoResearch Laboratories); HRP-labeled anti-mouse and anti-rabbit IgG (Jackson ImmunoResearch Laboratories).

Techniques: Transfection, Staining, Control, Incubation, Fluorescence, Expressing